How do I calculate peptide concentration after adding water?

Divide the amount in the vial by the volume of water you added. A 10 mg vial mixed with 2 mL gives 10 ÷ 2 = 5 mg/mL, which is the same as 5,000 mcg/mL.

How many units are in 1 mL on a U-100 syringe?

A U-100 insulin syringe is marked so that 100 units equal 1 mL. Each unit is 0.01 mL, so 0.25 mL reads as 25 units.

How do I convert mg to mcg?

Multiply milligrams by 1,000. 1 mg = 1,000 mcg, so 0.25 mg = 250 mcg. To go back, divide mcg by 1,000.

Can I convert units on the syringe back to an amount?

Yes. Divide the units by 100 to get mL, then multiply by the concentration. 20 units = 0.20 mL; at 5 mg/mL that holds 1 mg. The calculator does this when you type units into the units box.

Does adding more water change how much is in the vial?

No. The total in the vial stays the same. More water lowers the concentration, so the same amount takes up more volume and more units on the syringe.

Why is IU treated differently from mg?

IU measures biological activity, not weight, so there is no universal IU-to-mg rule. The calculator uses the IU count printed on your own vial label instead of guessing.

Does PeptideWhiz suggest doses?

No. PeptideWhiz never suggests or recommends an amount. Every number comes from you, and the tool only does the arithmetic. It is not medical advice.

Do I need an account to use the calculator?

No. The calculator is free and works without an account. An account only lets you save results to your stack and tracker.

Peptide Calculator

Q: What is Semaglutide?

Semaglutide is a GLP-1 receptor agonist. Semaglutide is a long-acting analogue of the gut hormone GLP-1 (glucagon-like peptide-1).

Q: Does this page suggest a Semaglutide dose?

No. The calculator dose field starts empty and PeptideWhiz never suggests, recommends or calculates an amount for anyone. The study table is a research record of what published trials reported, not a recommendation or protocol.

Q: Where do the study numbers on this page come from?

Each row quotes the abstract of a published human trial (5 trials, 2026 to 2026) word for word, as indexed in PubMed, with a link to the PubMed record. Nothing is rounded, combined or converted.

Q: How does the calculator work with a Semaglutide vial?

Pick Semaglutide in step 1 if you want it on your result card, then enter the vial amount printed on your own label and the water volume actually added. The tool divides one by the other for concentration and shows the matching U-100 syringe units for an amount you type. Pens and pre-filled syringes are already in liquid form and are not reconstituted.

Vial and water math for Semaglutide, with a research record of published human trials. Math only, no suggested amounts.

Free · no account needed

Compound (Optional)

Desired Amount Per Dose

units (U-100)

Vial Strength (Total mg of Your Vial)

Reconstitution Solution Added

Titration Schedule (Optional)

Results

Draw to

—units

Enter amount, vial and Reconstitution Solution

ConcentrationConc.

—mg/mL

Your dose

—mg

Doses / vial

—

More than 100 units (over 1 mL). This won't fit in a single U-100 syringe draw. Double-check your numbers or use more water / a smaller amount.

U-100 Syringe · 1 mL

Notes

PeptideWhizpeptidewhiz.app · research use only

Research use only (RUO). Results are math on the values you enter only, not a recommendation or prescription for human use. Not medical advice. Always verify your own measurements. Full disclaimer

About Semaglutide

Semaglutide is a long-acting analogue of the gut hormone GLP-1 (glucagon-like peptide-1). It binds the GLP-1 receptor, which is involved in insulin release after meals, stomach emptying and appetite signalling. A fatty side chain lets it bind albumin in the blood, which is why trials give it once a week rather than daily.

Research status

Semaglutide is the active ingredient in FDA-approved prescription medicines (Ozempic and Rybelsus for type 2 diabetes, Wegovy for chronic weight management). It remains one of the most studied compounds in metabolic research, with trials in diabetes, obesity, heart, kidney and liver outcomes.

How the math works for any vial (concentration, U-100 units, mg and mcg) is explained step by step on the main peptide reconstitution calculator page. Read the full What is Semaglutide? guide for mechanism, trials and conditions researched.

What published studies used

Research record, not a recommendation or protocol

These are published human trials of Semaglutide from the PeptideWhiz study library. The last column quotes each study’s PubMed abstract word for word, so it shows what that study reported giving its participants under medical supervision. It is not advice, and the calculator never fills these numbers in on its own: you can copy one only by tapping its button and confirming.

Study	Year	Journal	As reported in the abstract	PubMed
Efficacy and safety of once-weekly semaglutide 2·4 mg in Chinese adults with overweight or obesity (STEP 12): a randomised, double-blind, placebo-controlled, multicentre, phase 3b trial.	2026	The lancet. Diabetes & endocrinology	“Adults with a BMI of 24-<28 kg/m2 and at least one weight-related comorbidity, or a BMI of 28-<30 kg/m2, with or without type 2 diabetes, were randomly assigned (2:1) to once-weekly subcutaneous semaglutide 2·4 mg or placebo, plus lifestyle intervention, for 44 weeks.”	PMID 42575111
Insulin, Semaglutide and Dapagliflozin in Adults With Type 1 Diabetes: Design and Methods of Triple Therapy for Type 1 Diabetes (TTT1)-An International Phase 3 Clinical Trial.	2026	Diabetes, obesity & metabolism	“In Period 1, participants are randomized 2:1 (open label) for 26 weeks to semaglutide and insulin (uptitrated to 1.0 mg weekly) or standard insulin therapy.”	PMID 42473259
Short- and long-term effects of semaglutide 2.4 mg on energy intake, appetite, and food reward: a 60-week, double-blind randomized controlled trial.	2026	The American journal of clinical nutrition	“This 60-wk, double-blind trial randomly assigned 120 adults with overweight/obesity in a 3:2 ratio to semaglutide 2.4 mg or placebo.”	PMID 42323166
Effects of Semaglutide on Body Composition and GFR: A Prespecified Analysis of the SMART Trial.	2026	Clinical journal of the American Society of Nephrology : CJASN	“Participants were randomized to 24 weeks of semaglutide 2.4 mg/wk subcutaneously or matched placebo treatment.”	PMID 42308057
Cagrilintide-semaglutide (CagriSema) versus semaglutide or cagrilintide in people with type 2 diabetes (REIMAGINE 2): a double-blind, randomised, controlled, phase 3 study.	2026	The lancet. Diabetes & endocrinology	“The primary endpoint was change in HbA1c from baseline to week 68 with cagrilintide-semaglutide (2·4 mg each) versus semaglutide 2·4 mg in the full analysis set; safety was assessed in all participants who received at least one dose of study product.”	PMID 42251859

Disclaimer: this table is an educational research record. It does not recommend, suggest or endorse any amount, schedule or use, and it is not medical advice. Decisions about any medicine belong with a licensed clinician.

Semaglutide calculator FAQ

What is Semaglutide?

Semaglutide is a GLP-1 receptor agonist. Semaglutide is a long-acting analogue of the gut hormone GLP-1 (glucagon-like peptide-1).

Is Semaglutide approved?

Does this page suggest a Semaglutide dose?

No. The calculator dose field starts empty and PeptideWhiz never suggests, recommends or calculates an amount for anyone. The study table is a research record of what published trials reported, not a recommendation or protocol.

Where do the study numbers on this page come from?

Each row quotes the abstract of a published human trial (5 trials, 2026 to 2026) word for word, as indexed in PubMed, with a link to the PubMed record. Nothing is rounded, combined or converted.

How does the calculator work with a Semaglutide vial?

Pick Semaglutide in step 1 if you want it on your result card, then enter the vial amount printed on your own label and the water volume actually added. The tool divides one by the other for concentration and shows the matching U-100 syringe units for an amount you type. Pens and pre-filled syringes are already in liquid form and are not reconstituted.

Sources

Study rows: PubMed records linked in the table (abstracts checked against PubMed on October 31, 2026). Unit math: see the sources on the main calculator.

Reviewed by the PeptideWhiz team on October 31, 2026. For research and education only.