Physical Activity and Insulin Sensitivity Dynamics

What Was Tested

The trial (not phased) is built around Insulin (regular human).

The study investigates the role of one single bout of exercise in mediating enhancement of muscle insulin sensitivity following a single bout of exercise.

Furthermore, the study is aiming to elucidate the temporal development in insulin signaling, at the early timepoints of insulin stimulation that may be responsible for the enhanced muscle insulin sensitivity.

This will be investigated in young healthy males subjected to a one-legged knee-extensor exercise followed by a hyperinsulinemic-euglycemic clamp, a setup known to enhance muscle insulin sensitivity.

It had 1 arm (groups): Post-exercise insulin sensitivity (experimental).

Young healthy males will perform a single bout of knee-extensor exercise and insulin action is investigated 4 hours after cessation of exercise. Insulin action towards muscle glucose uptake will be investigated during a 120 min euglycemic hyperinsulinemic clamp.

The registry classes the interventions as other and procedure.

More on the Plan

The participant arrives fasting at 8:00 AM, having abstained from alcohol, nicotine, and caffeine for the past 24 hours and refrained from strenuous physical activity for the past 48 hours.

The participants are then subjected to a one-legged knee extension exercise performed by the participant from 9:00 AM to 10:00 AM.

Four hours after the exercise ends, a hyperinsulinemic-euglycemic clamp is commenced.

The clamp begins with a bolus injection of insulin, followed by a continuous infusion.

Who Took Part

Enrollment: 8 planned participants were the target.

It was open to men only aged from 22 years up to 35 years.

Healthy volunteers could join, not only people with the condition.

Examples of who could join: Healthy individuals (no known diseases). No use of medication. Aged 22-35 years. BMI between 28 and 35.

The full record lists 7 inclusion and 5 exclusion criteria; among the exclusions: failure to meet all inclusion criteria; physical activity level (e.g., running, cycling, fitness, etc.) exceeding 6 hours per week; acute illness within 2 weeks prior to the study days.

How the Study Was Run

The set-up was single-group and open-label.

Being open-label, the Insulin (regular human) study did not mask who received what.

Everyone received the same intervention, so there was no separate comparison group inside the trial.

It started in 2025-05-05 with completion listed for 2025-08-26.

It ran at 1 site in Denmark.

Its stated primary purpose is basic science.

What Was Measured

Researchers chose this as the main measure: Insulin stimulated muscle glucose uptake (time frame: Through study completion, approx. 1 year).

It also tracked 1 secondary outcome, for example Posttranslational modification of proteins in muscle biopsies.

What This Means in Plain Language

Put simply, this is a not phased trial of Insulin (regular human) in 8 people.

Its size is small, so on its own it can only give an early look.

It is still recruiting; no results are posted to the registry yet, so nothing here describes outcomes.

More context: the Insulin (regular human) compound page, all human trials.