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Metallothionein mRNA in monocytes and peripheral blood mononuclear cells and in cells from dried blood spots increases after zinc supplementation of men.
Plain-language guide to PMID 10958810 (2000): randomized controlled trial of Vitamin D, K2, Magnesium and Zinc in 16 men.
Key Facts
- PMID
- 10958810
- Journal
- The Journal of nutrition
- Year
- 2000
- Study type
- Randomized Controlled Trial
- Authors
- Cao J et al. (2 authors)
- DOI
- 10.1093/jn/130.9.2180
What This Study Did, in Plain Language
What Was Tested
The paper reports a study of Vitamin D, K2, Magnesium and Zinc, published as a randomized controlled trial.
A protocol involving 16 men and a 10-d supplementation period plus a 4-d postsupplementation period was used to examine the relative response of MT expression in erythrocytes, monocytes, peripheral blood mononuclear cells (PBMC) and cells from a dried blood spot (DBS).
Why It Was Studied
A specific, sensitive and reliable index for assessment of human zinc status has not been developed, and continues to present a considerable challenge for nutritionists in the trace element field.
The researchers have focused on metallothionein (MT) expression as a potential index.
Who Took Part
The abstract describes 16 men taking part.
PubMed indexes the participants as adults, men only.
How the Study Was Run
In design terms this was a randomized and placebo-controlled study.
Random assignment meant chance, not the researchers, decided who got Vitamin D, K2, Magnesium and Zinc and who got the comparison, which limits bias.
The comparison arm used a placebo, so changes seen with Vitamin D, K2, Magnesium and Zinc could be set against what happens without it.
What Was Measured
A specific, sensitive and reliable index for assessment of human zinc status has not been developed, and continues to present a considerable challenge for nutritionists in the trace element field.
PubMed files the paper under topics including Enzyme-Linked Immunosorbent Assay, Erythrocytes, Gene Expression, Metallothionein, Monocytes, Nutrition Policy and RNA, Messenger.
What This Means in Plain Language
The paper is a randomized and placebo-controlled study of Vitamin D, K2, Magnesium and Zinc in 16 men.
With fewer than 40 people, it is a small study, so it is better read as an early signal for further research than as a settled answer.
The publication is from 2000 (The Journal of nutrition), a journal based in United States.
More context: the Vitamin D, K2, Magnesium and Zinc compound page, all human trials.
Our Notes on the Compound
Vitamin D, K2, Magnesium and Zinc: Magnesium is described as required for AMPK to function.
Related Pages
Source: PubMed record. This explanation is written by PeptideWhiz from the public record. For research and education only. Not medical advice; no dosing information is given.