Expression of substance P, calcitonin gene-related peptide, β-endorphin and methionine-enkephalin in human dental pulp tissue after orthodontic intrusion: a pilot study

What Was Tested

The paper reports a study of CGRP (Calcitonin Gene-Related Peptide) and Substance P, published as a randomized controlled trial.

To determine the levels of two sensory neuropeptides (substance P [SP] and calcitonin gene-related peptide [CGRP]) and two endogenous opioids (methionine-enkephalin [Met-Enk] and β-endorphin [β-End]) in dental pulp tissue samples subjected to controlled orthodontic intrusive forces.

Who Took Part

The age groups tagged for this paper are children and teenagers, both men and women.

Sixteen healthy premolars were selected from eight patients who were undergoing extraction for orthodontic purposes.

Eight were randomly used as controls, and the other eight were assigned to an experimental group (controlled orthodontic intrusive forces applied for 24 hours).

How the Study Was Run

The design is described as randomized and pilot.

The participants were allocated by chance, so the CGRP (Calcitonin Gene-Related Peptide) group and the comparison group should start out alike.

It is described as a pilot, a small early test of whether a larger CGRP (Calcitonin Gene-Related Peptide) study would be practical.

After this period, teeth were extracted, and pulp samples were obtained.

All samples were processed to quantify the expression levels of SP, CGRP, Met-Enk, and β-End using commercial radioimmunoassay kits.

What Was Measured

Topics PubMed links to this paper include Bicuspid, Calcitonin Gene-Related Peptide, Dental Pulp, Enkephalin, Methionine, Neurogenic Inflammation, Neurotransmitter Agents and Opioid Peptides.

What This Means in Plain Language

The paper is a randomized and pilot study of CGRP (Calcitonin Gene-Related Peptide) and Substance P.

Its findings take up roughly 5 sentences of the abstract and are left out here on purpose; the source paper is the place to read them.

The publication is from 2014 (The Angle orthodontist), a journal based in United States.

More context: the CGRP (Calcitonin Gene-Related Peptide) compound page and the Substance P compound page, all human trials.