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Critical care and vitamin D status assessment: what about immunoassays and calculated free 25OH-D?
Plain-language guide to PMID 25020235 (2014): observational study of Vitamin D, K2, Magnesium and Zinc.
Key Facts
- PMID
- 25020235
- Journal
- Clinica chimica acta; international journal of clinical chemistry
- Year
- 2014
- Study type
- Observational Study
- Authors
- Rousseau AF et al. (9 authors)
- DOI
- 10.1016/j.cca.2014.07.007
What This Study Did, in Plain Language
What Was Tested
Researchers studied Vitamin D, K2, Magnesium and Zinc in this observational study publication.
The aim of this observational study was to evaluate effects of an acute hemodilution on vitamin D (VD) status.
Why It Was Studied
Interpretation of 25OH-D measurement during critical care (CC) may be problematic due to variations of binding protein concentrations (albumin, ALB, and vitamin D binding protein, VDBP).
Determination of free 25OH-D concentration may thus be relevant in CC patients.
Who Took Part
PubMed indexes the participants as young adults, adults, middle-aged adults and older adults, both men and women.
How the Study Was Run
Blood samples were obtained before (T1) and after a crystalloid load (T2) administered at anesthesia induction for minor surgery. 25OH-D was measured with LC-MS/MS and with 3 immunoassays (IA): DiaSorin Liaison, IDS iSYS and bioMérieux Vidas.
VDBP was measured with the R&D Elisa and ALB on Cobas.
Free 25OH-D was calculated using published formula.
Performances of the three AI were compared with LC-MC/MS using Bland-Altman analysis.
What Was Measured
Interpretation of 25OH-D measurement during critical care (CC) may be problematic due to variations of binding protein concentrations (albumin, ALB, and vitamin D binding protein, VDBP).
Topics PubMed links to this paper include Biomarkers, Calcifediol, Chromatography, Liquid, Critical Care, Critical Illness, Immunoassay and Mass Spectrometry.
Author keywords: Free 25-hydroxyvitamin D, Hemodilution and Vitamin D.
What This Means in Plain Language
This is an observational study of Vitamin D, K2, Magnesium and Zinc.
Results are reported in around 9 sentences of the abstract; we leave them to the paper itself rather than summarise them.
The publication is from 2014 (Clinica chimica acta; international journal of clinical chemistry), a journal based in Netherlands.
More context: the Vitamin D, K2, Magnesium and Zinc compound page, all human trials.
Our Notes on the Compound
Vitamin D, K2, Magnesium and Zinc: Magnesium is described as required for AMPK to function.
Related Pages
Source: PubMed record. This explanation is written by PeptideWhiz from the public record. For research and education only. Not medical advice; no dosing information is given.