The direct and indirect effects of kisspeptin-54 on granulosa lutein cell function

Plain-language guide to PMID 29206944 (2018): clinical trial study of Kisspeptin-10 and hCG (human chorionic gonadotropin).

Key Facts

PMID: 29206944
Journal: Human reproduction (Oxford, England)
Year: 2018
Study type: Clinical Trial
Authors: Owens LA et al. (12 authors)
DOI: 10.1093/humrep/dex357

What This Study Did, in Plain Language

What Was Tested

This clinical trial paper looks at Kisspeptin-10 and hCG (human chorionic gonadotropin).

What are the in vivo and in vitro actions of kisspeptin-54 on the expression of genes involved in ovarian reproductive function, steroidogenesis and ovarian hyperstimulation syndrome (OHSS) in granulosa lutein (GL) cells when compared with traditional triggers of oocyte maturation?

The use of kisspeptin-54 as an oocyte maturation trigger augmented expression of genes involved in ovarian steroidogenesis in human GL cells including, FSH receptor (FSHR), LH/hCG receptor (LHCGR), steroid acute regulatory protein (STAR), aromatase, estrogen receptors alpha and beta (ESR1, ESR2), 3-beta-hydroxysteroid dehydrogenase type 2 (3BHSD2) and inhibin A (INHBA), when compared to traditional maturation triggers, but did not alter markers of OHSS.

Who Took Part

By PubMed's age tags, the people studied were adults, women only.

Forty-eight women undergoing IVF treatment for infertility consented to kisspeptin-54 triggering and/or granulosa cell collection and were included in the study.

Twelve women received hCG, 12 received GnRH agonist and 24 received kisspeptin-54 to trigger oocyte maturation.

How the Study Was Run

Follicular fluid was aspirated and pooled from follicles during the retrieval of oocytes for IVF/ICSI.

GL cells were isolated and either RNA extracted immediately or cultured in vitro ± kisspeptin or hCG.

What Was Measured

Topics PubMed links to this paper include Cells, Cultured, Chorionic Gonadotropin, Gene Expression, Gonadotropin-Releasing Hormone, In Vitro Oocyte Maturation Techniques, Infertility and Kisspeptins.

Author keywords: IVF, granulosa lutein cells, maturation trigger and steroidogenesis.

What This Means in Plain Language

This is a clinical trial of Kisspeptin-10 and hCG (human chorionic gonadotropin).

The abstract goes on to report its results in about 8 sentences; this page does not restate them, so read the paper for the findings and their limits.

The publication is from 2018 (Human reproduction (Oxford, England)), a journal based in England.

More context: the Kisspeptin-10 compound page and the hCG (human chorionic gonadotropin) compound page, all human trials.

Our Notes on the Compounds

Kisspeptin-10: A hypothalamic signaling peptide that triggers GnRH, and through it LH and FSH.

hCG (human chorionic gonadotropin): An LH analog that drives Leydig-cell testosterone production.

Kisspeptin-54: The full-length 54-amino-acid kisspeptin, studied in people to trigger egg maturation in IVF; Kisspeptin-10 has its own page.

Source: PubMed record. This explanation is written by PeptideWhiz from the public record. For research and education only. Not medical advice; no dosing information is given.