Measurement of vitamin D in dried blood spots stored under different temperature conditions.

What Was Tested

The paper reports a study of Vitamin D, K2, Magnesium and Zinc, published as a clinical trial.

As part of the Survey of Health, Ageing and Retirement in Europe (SHARE) study, dried blood spot samples were obtained for measurement of potential biological biomarkers, among those vitamin D.

Unfortunately, no studies describe the impact of high temperatures on dried blood spot samples and vitamin D measurements.

Who Took Part

The age groups tagged for this paper are middle-aged adults, older adults and people over 80, both men and women.

Capillary samples were collected on dried blood spot cards from 40 outpatients (median age 78 years) along with venous blood samples.

To mimic the different environmental and temporal challenges during collection and shipment until final storage in the SHARE study, dried blood spot cards were stored at different temperatures, at time span and with/without freeze-thaw.

How the Study Was Run

Vitamin D concentrations in venous plasma samples were measured by conventional immunoassay (on Architect i2000SR), while vitamin D concentrations in dried blood spot samples were measured using LC-MS/MS with a well-described extraction method and with relevant calibration and comparison with a reference method.

What Was Measured

As part of the Survey of Health, Ageing and Retirement in Europe (SHARE) study, dried blood spot samples were obtained for measurement of potential biological biomarkers, among those vitamin D.

Unfortunately, no studies describe the impact of high temperatures on dried blood spot samples and vitamin D measurements.

The subject headings attached to it are Blood Preservation, Chromatography, High Pressure Liquid, Dried Blood Spot Testing, Tandem Mass Spectrometry and Vitamin D.

The authors tagged it with the keywords Clinical studies and laboratory methods.

What This Means in Plain Language

This is a clinical trial of Vitamin D, K2, Magnesium and Zinc.

The abstract goes on to report its results in about 4 sentences; this page does not restate them, so read the paper for the findings and their limits.

The publication is from 2021 (Annals of clinical biochemistry), a journal based in England.

More context: the Vitamin D, K2, Magnesium and Zinc compound page, all human trials.