A Plant-Based Dietary Supplement Exhibits Significant Effects on Markers of Oxidative Stress, Inflammation, and Immune Response in Subjects Recovering from Respiratory Viral Infection: A Randomized, Double-Blind Clinical Study Using Vitamin C as a Positive Control.

What Was Tested

This randomized controlled trial looks at Copper (Supplement).

Respiratory viruses continue to present serious health challenges to human wellness.

The simultaneous over-stimulation of several reactive oxygen species (ROS) pathways and concurrent suppression of bioavailable Nitic Oxide (NO) contribute to an immune disbalance that can lead to cellular oxidative distress and an excessive inflammatory response.

Who Took Part

According to the abstract, 28 individuals were involved.

PubMed indexes the participants as adults and middle-aged adults, both men and women.

How the Study Was Run

The design is described as randomized and double-blind.

The individuals were allocated by chance, so the Copper (Supplement) group and the comparison group should start out alike.

As a double-blind Copper (Supplement) study, the assignments were hidden from both participants and investigators until the end.

Changes in the levels of bioavailable NO (measured as circulating NOHb) were assessed, as well as the ex vivo cellular formation of mitochondrial, NOX2-, iNOS-, and TNFα-dependent ROS.

All parameters were measured in real time before ingestion (baseline), and then at 30, 60, 120, and 180 min after administration.

ROS were measured using a portable electron paramagnetic resonance (EPR) spectrometer.

What Was Measured

Inflammatory, immunity (hsCRP and TNFα plasma levels), interleukin (IL1, IL6, IL8, and IL10), cytokine (IFNγ, TNFα, and NF-κB), and immunoglobulin (IgA, IgM, IgG, and IgE) profiles were also followed.

PubMed files the paper under topics including Dietary Supplements, Oxidative Stress, Ascorbic Acid, COVID-19, Inflammation, Biomarkers and COVID-19 Drug Treatment.

The authors tagged it with the keywords FMD, ROS, cellular metabolic activity (CMA), circulating NOHb, dietary supplement PB-blend and endothelial dysfunction.

What This Means in Plain Language

The paper is a randomized and double-blind study of Copper (Supplement) in 28 individuals.

With fewer than 40 people, it is a small study, so it is better read as an early signal for further research than as a settled answer.

The publication is from 2025 (International journal of molecular sciences), a journal based in Switzerland.

More context: the Copper (Supplement) compound page, all human trials.