Investigation of the Effect of Vitamin D Supplementation on Apical Lesion Healing Rate: Prospective, Randomised Clinical Trial.

What Was Tested

This randomized controlled trial looks at Vitamin D, K2, Magnesium and Zinc.

This study was conducted to evaluate the effect of maintaining optimal serum vitamin D levels through supplementation on the healing rate and quality of newly formed bone at 3, 6, 9, and 12 months in teeth with periapical lesions following endodontic treatment.

Additionally, TNF-α levels were measured to investigate whether the potential healing effect was related to the anti-inflammatory properties of vitamin D.

Who Took Part

PubMed indexes the participants as adults and middle-aged adults, men only.

Seventy-three male patients with mature mandibular molars presenting with periapical lesions and meeting the inclusion criteria were allocated into two groups: the vitamin D group (n = 37) and the control group (n = 36).

All patients received root canal treatment according to a standardised endodontic protocol.

How the Study Was Run

The design is described as randomized and parallel-group.

Because people in this Vitamin D, K2, Magnesium and Zinc study were split into groups at random, differences between the groups are less likely to reflect who happened to join each one.

The Vitamin D, K2, Magnesium and Zinc group and the comparison group ran side by side rather than switching.

This study was designed as a parallel, triple-blind, randomised controlled clinical trial registered at the Clinical Trials Registry.

The vitamin D group received oral vitamin D supplements to raise serum 25(OH)D level to the optimal range (30-50 ng/mL), while the control group, with insufficient serum 25(OH)D levels (20-30 ng/mL), did not receive any supplementation.

Periapical radiographs (pre-op, 3, 6, 9, 12 months), serum 25(OH)D (chemiluminescence), and TNF-α (ELISA) levels were assessed.

What Was Measured

The subject headings attached to it are Vitamin D, Dietary Supplements, Wound Healing, Tumor Necrosis Factor-alpha, Root Canal Therapy and Periapical Diseases.

Author keywords: TNF‐α, endodontics, periapical periodontitis and root canal treatment.

What This Means in Plain Language

The paper is a randomized and parallel-group study of Vitamin D, K2, Magnesium and Zinc.

Results are reported in around 4 sentences of the abstract; we leave them to the paper itself rather than summarise them.

The publication is from 2026 (International endodontic journal), a journal based in England.

More context: the Vitamin D, K2, Magnesium and Zinc compound page, all human trials.