Co-supplementation of zinc and probiotic on oxidative stress markers and sleep quality index in irritable bowel syndrome patients: a double-blind randomized controlled trial.

What Was Tested

Researchers studied Vitamin D, K2, Magnesium and Zinc in this randomized controlled trial publication.

Irritable bowel syndrome (IBS) is a functional gastrointestinal (GI) disorder that is related to impaired gut microbiota (GM) composition and sleep disorders.

Zinc and probiotics play key roles in gut barrier integrity maintenance and microbiota modulation.

Who Took Part

The study group was 84 participants.

The age groups tagged for this paper are adults and middle-aged adults, both men and women.

How the Study Was Run

In design terms this was a randomized, double-blind, placebo-controlled and parallel-group study.

The participants were allocated by chance, so the Vitamin D, K2, Magnesium and Zinc group and the comparison group should start out alike.

Neither the participants nor the staff measuring them knew who was on Vitamin D, K2, Magnesium and Zinc, so expectations could not easily colour the readings.

A placebo (an inactive look-alike) gave the Vitamin D, K2, Magnesium and Zinc group a baseline to be compared against.

The Vitamin D, K2, Magnesium and Zinc group and the comparison group ran side by side rather than switching.

After 10-12 h overnight fasting, serum zinc, total antioxidant capacity (TAC), total oxidant status (TOS), malondialdehyde (MDA) levels, superoxide dismutase (SOD) activity as well as sleep quality were assessed by validated methods.

Oxidative stress index (OSI) was also calculated.

What Was Measured

Irritable bowel syndrome (IBS) is a functional gastrointestinal (GI) disorder that is related to impaired gut microbiota (GM) composition and sleep disorders.

PubMed files the paper under topics including Probiotics, Oxidative Stress, Zinc, Irritable Bowel Syndrome, Sleep Quality, Biomarkers and Dietary Supplements.

What This Means in Plain Language

The paper is a randomized, double-blind and placebo-controlled study of Vitamin D, K2, Magnesium and Zinc in 84 participants.

Results are reported in around 5 sentences of the abstract; we leave them to the paper itself rather than summarise them.

The publication is from 2026 (European journal of nutrition), a journal based in Germany.

More context: the Vitamin D, K2, Magnesium and Zinc compound page, all human trials.